WE ARE HIRING

Research in the Gale laboratory is focused on understanding the processes that trigger and control innate immunity and inflammation to program the immune response against RNA virus infection, and to define the virus-host interactions that control viral replication and the outcome of infection and immunity. We are also focused on defining the systems biology and innate immune interactions of acute and chronic microbial infection toward building interventions to fight disease and improve global health.
The Research Scientist 2 will organize and execute highly collaborative NHP ZIKV studies; they will also work to establish high-throughput assays to support the viral sequencing research in the Gale lab.
You can find the complete job posting and application at this link.

 CUSTOM SEQUENCING SOLUTIONS

Seattle Genomics, a service core at the University of Washington, offers next-generation sequencing and data analysis solutions.
The center is a component of the Washington National Primate Research Center and is operated by the Division of NHP Systems Biology in the Gale laboratory.

Services and Rates

We offer the following:

Illumina NextSeq 500
Single Cell isolation, barcoding & library prep
RNA library preparation and QC
Illumina MiSeq
Bioinformatics analysis and support
RNA Isolation and QC

The below costs are calculated using the pricing associated with payment using a UW budget with Federal Funds.

mRNA-seq

For example, for 20 samples to have RNA isolated from tissue, mRNA-Seq libraries constructed and sequenced on our NextSeq 500 using a high output 150 cycle (paired end 75bp), the total cost would be $7,675.21, which averages to $383.76 per sample including taxes. This run will produce approximately 20 million reads per sample.

Total RNA-seq

For example, for 20 samples to have RNA isolated from tissue, Total RNA-Seq libraries constructed and sequenced on our NextSeq 500 using a high output 150 cycle (paired end 75bp), the total cost would be $12,102.42 which averages to $605.12 per sample including taxes. This run will produce approximately 40 million reads per sample.

Custom applications REQUIRE consultation to discuss project.


Sequencing Run Costs

MiSeq RunsUWUW (Non-Federal)External
per sequencing run
per sequencing runper sequencing run
v2 300 Cycle$1,490.50$1,534.76Contact us
v3 150 Cycle$1,326.34$1,365.73Contact us
v3 600 Cycle$2,056.54$2,117.61Contact us

Next Seq 500 RunsUWUW (Non-Federal)External
per sequencing runper sequencing runper sequencing run
MID Output 150 Cycle$1,548.41$1,594.39Contact us
MID Output 300 Cycle$2,325.16$2,394.20Contact us
HIGH Output 75 Cycle$2,028.46$2,088.69Contact us
HIGH Output 150 Cycle$3,625.47$3,733.12Contact us
HIGH Output 300 Cycle$5,635.35$5,802.68Contact us

RNA Extraction and QC Costs

ItemUWUW (Non-Federal)External
per sampleper sampleper sample
RNA Isolation - General$49.42$50.89Contact us
RNA Isolation - Blood$57.67$59.38Contact us
Nucleic Acid QC$23.46$24.15Contact us
Homogenation - Nucleic Acid$30.00$30.89Contact us
Prepared Library QC (Qubit & Bioanalyzer) (up to 10 samples)$81.68$81.68Contact us
qPCR - 96 well plate$242.20$242.20Contact us

RNA-seq

ItemUWUW (Non-Federal)External
per sampleper sampleper sample
mRNA Library Preparation (≥ 20 samples)$138.79$142.91Contact us
Total RNA-seq Library Preparation (≥ 20 samples)$178.88$184.19Contact us
mRNA Hyper + Globin Removal Library Prep (≥ 20 samples)$182.21$187.62Contact us
Ultra-Low Input Library Preparation$234.82$241.79Contact us
Swift Normalase Amplicon Panel (SNAP) SARS-CoV-2 Panel$127.39$131.21Contact us

Single Cell

ItemUWUW (Non-Federal)External
Cell Isolation & Barcoding - 1 sample$2,152.58$2,216.49Contact us
Cell Isolation & Barcoding - 2 samples$3,720.93$3,831.42Contact us
Cell Isolation & Barcoding - 3 samples$5,298.64$5,455.98Contact us
Cell Isolation & Barcoding - 4 samples$6,866.99$7,070.90Contact us
per sampleper sampleper sample
Cell Isolation & Barcoding - 5 to 8 samples$1,659.04$1,708.30Contact us
RNAseq Library Preparation - 1 to 4 samples$392.21$403.86Contact us
RNAseq Library Preparation - 5 to 8 samples$219.52$226.03Contact us

NanoString

ItemUWUW (Non-Federal)External
per sampleper sampleper sample
NanoString nCounter CodeSet (if available)$25.00$25.75Contact Us
NanoString nCounter Preparation & Digital Analyzer$100.00$103.00Contact Us

Data Analysis and Delivery

For file delivery and analysis, we offer multiple options to suit the specific needs of the end user.

A link will be provided to download all of the raw (FASTQ) files and QC summaries.  This, as well as a summary of read and run QC is provided as part of the sequencing cost.
For customers desiring a more complex and comprehensive bioinformatics analysis and processing a free consultation up to 2 hours will be provided prior to any sample processing or sequencing.  This consultation is mandatory for this type of analysis to ensure the numbers of samples and comparisons are adequate for the types of analysis desired


Analysis tier 1:

Mapping to a supplied genome/reference with or without annotation, and results in a delivery of a BAM/SAM file to the customer.  This BAM/SAM file can then be used for the next steps of data processing and analysis.


Analysis tier 2:

Targeted to users without sufficient computing resources for data processing, but which have expertise and access to bioinformatics tools for downstream functional analysis and interpretation. For RNA-seq data, raw data matrix as well as differential expression analysis will be provided to the user. For host or pathogen genetic analysis, alignments and variant files will be provided. Users will be responsible for downstream analysis.


Analysis tier 3:

Targeted to users without sufficient computing or expertise in downstream analysis and interpretation. These interactions will be more collaborative in nature. We will consult with the user to define a plan that will include any required aspects of a project, from library preparation to downstream analysis.   For RNA-seq, the user will be provided with transcript quantification and the raw data matrix as well as functional analysis based upon their specific biological questions. If necessary, we will also provide de novo assembly of transcriptome or virus genome where a specified reference is not available.


Pricing (up to 24 samples):

Hourly Rates: $99.33 / hour (Internal, Federal) or $102.28 / hour (Internal, Non-Federal). Please contact us for external rates.

Analysis Tier 1:  30 hours.
Analysis Tier 2:  50 hours.
Analysis Tier 3:  This will require a custom price quote. There would be a minimum of 90 hours charged.


Requesting Sequencing Services

  1. Obtain a quote. Begin this process using our online form.
  2. Prepare your samples or libraries for submission.
  3. Ship or drop of your samples to the address below.
  4. We will e-mail confirmation of receipt and what services will be performed.
  5. Await a sequencing report at the end of sequencing project, with directions on how to get data from BaseSpace if not locally delivered.

We will store locally all sequence and run data for 3 months.

 

Sample and Library Submission Guidelines

Locally libraries can be delivered to the adress below. For shipping please contact us first for guidelines and further instructions.

Shipping Samples

Seattle Genomics
Room F460
Building F
750 Republican St.
Seattle, WA 98109-4766
(206)897-6068





Request a quote


Your Name*
Your Email*
If Academic , Principal Investigator(PI)
Company
How many samples?
< 2020 or more
If only requesting sequencing, which platform?
NextSeqMiSeq
Please check which services you need below.
RNA IsolationDNA IsolationNucleic Acid QC
What type of library preparation is needed?
mRNA-seqTotal RNA-seq16SOther
Will you be requiring analysis/Bioinformatics support?
Level of Analysis (See above for description)
Additional comments:

* required

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