WE ARE HIRING
We are hiring a Research Scientist 2 in the Gale Lab in the Department of Immunology located at University of Washington’s South Lake Union campus in our sequencing core.
Your tasks will focus primarily on performing high throughput sample processing including RNA/DNA isolation and quality control (QC), construction and QC of sequencing libraries (mainly mRNA-seq and total RNA-seq libraries), and quantitative RT-PCR assays. Samples originate from in vitro cell cultures, animal models, and human clinical specimen.
CUSTOM SEQUENCING SOLUTIONS
Seattle Genomics, a service core at the University of Washington, offers next-generation sequencing and data analysis solutions.
The center is a component of the Washington National Primate Research Center and is operated by the Division of NHP Systems Biology in the Gale laboratory.
Services and Rates
We offer the following:
The below costs are calculated using the pricing associated with payment using a UW budget with Federal Funds.
For example, for 20 samples to have RNA isolated from tissue, mRNA-Seq libraries constructed and sequenced on our NextSeq 500 using a high output 150 cycle (paired end 75bp), the total cost would be $7,451.67, which averages to $372.58 per sample including taxes. This run will produce approximately 20 million reads per sample.
For example, for 20 samples to have RNA isolated from tissue, Total RNA-Seq libraries constructed and sequenced on our NextSeq 500 using a high output 150 cycle (paired end 75bp), the total cost would be $11,749.94 which averages to $587.50 per sample including taxes. This run will produce approximately 40 million reads per sample.
Custom applications REQUIRE consultation to discuss project.
Sequencing Run Costs
|MiSeq Runs||UW||UW (Non-Federal)||External|
|per sequencing run||per sequencing run||per sequencing run
|v2 300 Cycle||$1,447.09||$1,490.06||Contact us|
|v3 150 Cycle||$1,287.71||$1,325.95||Contact us|
|v3 600 Cycle||$1,996.64||$2,055.93||Contact us|
|Next Seq 500 Runs||UW||UW (Non-Federal)||External|
|per sequencing run||per sequencing run||per sequencing run|
|MID Output 150 Cycle||$1,503.31||$1,547.95||Contact us|
|MID Output 300 Cycle||$2,257.44||$2,324.47||Contact us|
|HIGH Output 75 Cycle||$1,969.38||$2,027.85||Contact us|
|HIGH Output 150 Cycle||$3,519.87||$3,624.39||Contact us|
|HIGH Output 300 Cycle||$5,471.21||$5,633.67||Contact us|
|per pooled sample||per pooled sample||per pooled sample|
|Sequencing Library QC||$213.77||$220.12||Contact us|
RNA Extraction and QC Costs
|per sample||per sample||per sample|
|RNA Isolation - General||$47.98||$49.41||Contact us|
|RNA Isolation - Blood||$55.99||$57.65||Contact us|
|Nucleic Acid QC||$22.77||$23.45||Contact us|
|Homogenation - Nucleic Acid||$29.13||$29.99||Contact us|
|per sample||per sample||per sample|
|mRNA Library Preparation||$134.75||$138.75||Contact us|
|Total RNA-seq Library Preparation||$173.67||$178.83||Contact us|
|mRNA Hyper + Globin Removal Library Prep||$176.90||$182.16||Contact us|
|Ultra-Low Input Library Preparation||$227.98||$234.75||Contact us|
Data Analysis and Delivery
For file delivery and analysis, we offer multiple options to suit the specific needs of the end user.
A link will be provided to download all of the raw (FASTQ) files and QC summaries. This, as well as a summary of read and run QC is provided as part of the sequencing cost.
For customers desiring a more complex and comprehensive bioinformatics analysis and processing a free consultation up to 2 hours will be provided prior to any sample processing or sequencing. This consultation is mandatory for this type of analysis to ensure the numbers of samples and comparisons are adequate for the types of analysis desired
Analysis tier 1:
Mapping to a supplied genome/reference with or without annotation, and results in a delivery of a BAM/SAM file to the customer. This BAM/SAM file can then be used for the next steps of data processing and analysis.
Analysis tier 2:
Targeted to users without sufficient computing resources for data processing, but which have expertise and access to bioinformatics tools for downstream functional analysis and interpretation. For RNA-seq data, raw data matrix as well as differential expression analysis will be provided to the user. For host or pathogen genetic analysis, alignments and variant files will be provided. Users will be responsible for downstream analysis.
Analysis tier 3:
Targeted to users without sufficient computing or expertise in downstream analysis and interpretation. These interactions will be more collaborative in nature. We will consult with the user to define a plan that will include any required aspects of a project, from library preparation to downstream analysis. For RNA-seq, the user will be provided with transcript quantification and the raw data matrix as well as functional analysis based upon their specific biological questions. If necessary, we will also provide de novo assembly of transcriptome or virus genome where a specified reference is not available.
Pricing (up to 24 samples):
Hourly Rates: $96.44 / hour (Internal, Federal) or $99.30 / hour (Internal, Non-Federal). Please contact us for external rates.
Analysis Tier 1: 30 hours.
Analysis Tier 2: 50 hours.
Analysis Tier 3: This will require a custom price quote. There would be a minimum of 90 hours charged.
Requesting Sequencing Services
- Obtain a quote. Begin this process using our online form.
- Prepare your samples or libraries for submission.
- Ship or drop of your samples to the address below.
- We will e-mail confirmation of receipt and what services will be performed.
- Await a sequencing report at the end of sequencing project, with directions on how to get data from BaseSpace if not locally delivered.
We will store locally all sequence and run data for 3 months.
Sample and Library Submission Guidelines
Locally libraries can be delivered to the adress below. For shipping please contact us first for guidelines and further instructions.
750 Republican St.
Seattle, WA 98109-4766